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As described previously 34, iron chelated IMAC beads were prepared from Ni NTA superflow agarose beads Qiagen, 1018611 that were stripped of nickel with 100 mmol L EDTA and incubated in an aqueous solution of 10 mmol L FeCl 3 Sigma, 451649 <a href=https://enhanceyourlife.mom/>priligy uk</a>